| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Marcyan, Maya G (13)
|
NITTSC |
29.60 |
29.84 |
0.24 |
| 2 |
Taylor, Payton E (14)
|
NITTSC |
33.05 |
32.13 |
-0.92 |
| 3 |
Hill, Amanda L (14)
|
NITTSC |
|
33.40 |
- |
| 4 |
Clouser, Kristiana I (14)
|
NITTSC |
36.46 |
34.06 |
-2.40 |
| 5 |
Denham, Myanna M (13)
|
NISTAR |
37.36 |
35.88 |
-1.48 |
| 6 |
Bembenista, Julia C (13)
|
NITWST |
38.37 |
35.93 |
-2.44 |
| 7 |
Daley, Adeline R (13)
|
NISTAR |
|
39.21 |
- |
| 8 |
Korsh, Rebecca M (13)
|
NITTSC |
40.39 |
39.53 |
-0.86 |
| 9 |
Eisenmann, Abigail E (13)
|
NISTAR |
42.74 |
40.67 |
-2.07 |
|
Killion, Natalie A (13)
|
NICLSC |
29.17 |
NS |
- |
|
Tothero, Katherine M (13)
|
NISTAR |
47.85 |
NS |
- |